| MDM2 forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is overexpressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer. |
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| Catalog # | M2800-03A |
| Applications | Suitable for use in Immunohistochemistry (frozen, paraffin and resin), Immunoprecipitation and Western Blot. Other applications have not been tested. |
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| Recommended Dilutions: |
| Immunohistochemistry (Paraffin): 1:200-1:500; Requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA, pH 8.0, is recommended. |
| Western Blot: 1:1000 |
| Optimal working dilutions to be determined by researcher. |
| Hybridoma | Mouse myeloma X63-Ag8.653 with BALB/c spleen cells. |
| Recommended Positive Control | Human Breast Carcinoma |
| Recommended Negative Control | I1904-78R: IgG1 Murine Negative Control |
| Recommended Secondary Antibodies | I1904-14C: IgG, Fc (HRP) Pab Gt x Mo |
| I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2649 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa154-167, CSRPSTSSRRRAISE, of human MDM2. |
| Specificity | Recognizes human MDM2 protein (90kD). Species Crossreactivity: Mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
