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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -MDM2 (Murine Double Minute-2)

Anti -MDM2 (Murine Double Minute-2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
MDM2 forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is overexpressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
Catalog #M2800-03A
ApplicationsSuitable for use in Immunohistochemistry (frozen, paraffin and resin), Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:200-1:500; Requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA, pH 8.0, is recommended.
Western Blot: 1:1000
Optimal working dilutions to be determined by researcher.
HybridomaMouse myeloma X63-Ag8.653 with BALB/c spleen cells.
Recommended Positive ControlHuman Breast Carcinoma
Recommended Negative ControlI1904-78R: IgG1 Murine Negative Control
Recommended Secondary AntibodiesI1904-14C: IgG, Fc (HRP) Pab Gt x Mo
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2649
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa154-167, CSRPSTSSRRRAISE, of human MDM2.
SpecificityRecognizes human MDM2 protein (90kD). Species Crossreactivity: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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