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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -MDM2 (Murine Double Minute-2)

Anti -MDM2 (Murine Double Minute-2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #M2800-03B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2-4ug/ml detects MDM2 in RIPA lysates from HeLa cells. Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2800-03B
(2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide (CAISETEENSDELSGERQRKRHK)
corresponding to amino acids 164-185 of human MDM2 (mouse double minute 2) with an N-terminal cysteine added for conjugation purposes. The immunizing peptide is identical in both MDM2 isoforms, p90MDM2 and p76MDM2.
SpecificityRecognizes MDM2, Mr 76kD. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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