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You are here:Home » Antibodies » Abs to Ubiquitin » Anti -MDM2 (Murine Double Minute-2)

Anti -MDM2 (Murine Double Minute-2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
The MDM2 (murine double minute 2) proto-oncogene was originally identified as an amplified gene in a mouse tumor cell line. Overexpression of MDM2 was shown to produce tumors in athymic mice. In a separate set of studies, the 90kD MDM2 was found to bind and inactivate the transcriptional activity of the p53 protein. p53 was also identified as the major target for MDM2 during embryonic development by virtue of the fact that the lethal effects produced by knocking out MDM2 could be reversed by the simultaneous deletion of p53. The MDM2 gene itself is a transcriptional target for p53, and induction of p53 transcriptional activity results in increased MDM2 mRNA and protein levels. It appears that a MDM2 p53 feedback loop serves to keep the growth suppressive functions of p53 in check during the normal cell cycle. Recent studies have implicated MDM2 in regulating cell proliferation via p53-independent pathways. This is based on evidence that MDM2 can interact with Rb, E2F-1 and DP1.
Catalog #M2800-05
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: 1-5ug/ml
Western Blot: 0.5-2ug/ml (chemiluminescence detection).
Immunohistochemistry (Frozen): 1-5ug/ml
Immunohistochemistry (FFPE): 1-5ug/ml. Staining requires heat induced epitope retrieval (HIER) pretreatment.
Immunoprecipitation: 1ug/sample (autoradiographic detection).
Optimal dilutions to be determined by the researcher.
Positive Control (IF, WB, IP)OsA-CL, MCF-7 and HeLa cell lysates
Negative Control (IF, WB, IP):
HOS cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No3G187
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to human MDM2 protein. Epitope: Located within aa26-169 of the human protein.
SpecificityRecognizes the human MDM2 protein. Does not react with mouse MDM2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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