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Anti -MDM2, phosphorylated (Ser166) (Murine Double Minute-2)
Anti -MDM2, phosphorylated (Ser166) (Murine Double Minute-2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1) via Akt-mediated MDM2 phosphorylation at Ser166 and Ser186 (2,3). Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53 (4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3). Specificity/Sensitivity: Phospho-MDM2 (Ser166) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 166. | | | Catalog # | M2800-06 | | Applications | Suitable for use in Western Blotting. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. | | Purity | Purified by protein A and peptide affinity chromatography. | | Immunogen | Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser166 of human MDM2. | | Specificity | Detects endogenous levels of MDM2 only when phosphorylated at serine 166. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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