| MRP1 is a 190kD transmembrane phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines and tumors (1-5). |
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| Catalog # | M2825-13C |
| Applications | Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:100-1:500 (Anti mouse IgG labeled with HRP). |
| Immunocytochemistry: 1:20-1:50. [PBS, 0.5% paraformaldehyde-fixed or 70% methanol-fixed cytospin preparations (2)]. |
| Immunohistochemistry: FFPE sections following antigen retrieval by microwave heating in citrate (5). |
| Flow Cytometry: 1:20. Cells fixed in PBS, 0.5% paraformaldehyde for 30 minutes, followed by anti-mouse IgG labeled with FITC. Note: Block and wash with solution containing 0.1% Tween. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. Protect from light. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H406 |
| Host | Mouse |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE). |
| Purity | Purified by affinity chromatography. |
| Immunogen | Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1(1-3). |
| Specificity | Recognizes human MDR protein with a defined linear epitope (aa918-924) of MRP1. Does not crossreact with human MDR1, MDR3, MRP2, MRP3, MRP4, MRP5 or MRP6 gene products. Does not crossreact with mouse mrp1. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
