Anti -MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B
(MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1,
PGY1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH FC IF |
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| Shows no crossreactivity with the human MDR3 product. Its epitope localizes in the extracellular amino terminal half of P-glycoprotein (at the third extracellular loop of the molecule). This epitope is resistant to formalin fixation and periodate oxidation1 and is different from those of other commonly known Mabs to P-glycoprotein, such as C219, JSB-1, and MRK16, which recognize an intracellular and extracellular epitope of P-glycoprotein, respectively. Detects soluble P-glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P-glycoprotein.2 Soluble P-glycoprotein is also detected in extracellular fluids of cancer patients, such as malignant ascites and serum. The Mr of soluble P-glycoprotein is the same as that of membrane bound P-glycoprotein. | | | Catalog # | M2825-14B | | Applications | Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested. | | Recommended Dilutions | Flow Cytometry: Fixed cells only. | | Western Blot: 1-2ug/ml for 2hrs at RT | | Immunohistochemistry: Formalin/paraffin | | Optimal dilutions to be determined by the researcher. | | Positive Control | Drug resistant cells, Kidney or breast carcinoma. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 0.N.505 | | Host | Mouse | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Human and hamster drug-resistant viable cells followed by crude plasma membranes. Cellular Localization: Cell membrane. | | MW | 170kD. Epitope: Extracellular amino half | | Specificity | Recognizes soluble P-Glycoprotein in cultured media of viable adriamycin-resistant cells, whereas those of the drug-resistant parent cells contain no detectable level of soluble P-Glycoprotein. | | Species crossreactivity | Human and chinese hamster. Does not react with mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for Anti -MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1) |
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