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You are here:Home » Antibodies » Abs to Multi Drug Resistance (MDR) » Anti -MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B (MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1, PGY1)

Anti -MDR1 (Multi Drug Resistance Protein 1, ABC20, ATP Binding Cassette Subfamily B
(MDR/TAP) Member 1, ABCB1, CD243, CLCS, GP170, P-Glycoprotein, P-gp, P Glycoprotein 1,
PGY1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC FC
P-glycoprotein is encoded by the MDR1 (ABCB1) gene. It is a member of the ABC superfamily of ATP-binding cassette transporters. Expression of P-glycoprotein is associated with ATP-dependent transmembrane efflux of chemically diverse compounds from the cell. It is the major component of multidrug resistance in human tumors.
Catalog #M2825-14Z
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Cell permeabilization required
Western Blot: 1:50-1:100
Immunohistochemistry (Paraffin, Frozen): For paraffin sections, dilute 1:25 for biotin based detection. Heat-induced antigen retrieval required. The antibody should be incubated for 60 minutes at RT.
Immunocytochemistry: Cytospin preparations
Optimal dilutions to be determined by the researcher.
Positive Controls
Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.
Tissue Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretory epithelium) are recommended.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D562
HostMouse
ConcentrationNot Determined
FormSupplied as a liquid in PBS, 1% BSA, 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSDS-solubilized plasma membranes of a MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.
SpecificityRecognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein at ~170kD and MDR3 P-glycoprotein at ~140kD; not species specific.
Crossreactivity ProteinsA ~200kD protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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