| Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family. The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs. MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate. MRP2 is predominately expressed on the apical membranes in the liver and kidney proximal tubules. It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes. |
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| Applications: |
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| Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested. |
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| Catalog # | M2825-17F |
| Recommended Dilution | Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations. |
| Immunohistochemistry (frozen): 1:20 dilution on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. |
| Immunohistochemistry (paraffin): Pretreatment with 0.01M citric acid, pH 6.0 in ddH2O at 100°C for 5 min 3 times. After washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP). |
| Note: Mouse samples are NOT suitable for use in IHC. |
| Western Blot: 1:20-1:50. Use an IgG (HRP) anti-mouse- as secondary antibody. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 6D566 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~0.25mg/ml (Total protein) |
| Form | Supplied as a liquid, 0.7% BSA, 0.1% sodium azide. |
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| Purity | Supernatant |
| Immunogen | Recombinant corresponding to aa1339-1541 of the C-terminal of rat MDR2. |
| Specificity | Recognizes an internal epitope of rat MRP2. Species crossreactivity: human and mouse (Does not in react with mouse MRP2 in IHC). Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
