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You are here:Home » Antibodies » Abs to Enzymes, Glycosidase » Anti -MGEA5 (MEA5, FLJ11229, FLJ23355, KIAA0679, OGA, Beta-N-acetylhexosaminidase, Hexosaminidase C, O-GlcNAcase)

Anti -MGEA5 (MEA5, FLJ11229, FLJ23355, KIAA0679, OGA, Beta-N-acetylhexosaminidase,
Hexosaminidase C, O-GlcNAcase)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (1). MGEA5 also possesses hyaluronidase activity (2).
Catalog #M2840-01
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Not suitable for Flow Cytometry or Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:10,000-1:50,000
Immunohistochemistry: 1:100-1:250 Requires heat mediated ntigen retrieval
Immunoprecipitation: 1:100-1:500
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa, BxPC-3, JAR, fetal brain lysates, human kidney lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
Clone NoEPR7154(B)
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues in human MGEA5.
SpecificityRecognizes human MGEA5 at ~130kD. Species crossreactivity: mouse. Does not react with rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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