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You are here:Home » Molecular Biology » MB-Protein Kinase Inhibitors » MEK1 Inhibitor (PD98059, 2-amino-3-methoxyflavone)

MEK1 Inhibitor (PD98059, 2-amino-3-methoxyflavone)

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Specifications

PD98059 (MEK1 Inhibitor) has been shown to act in vivo as a highly selective inhibitor of MEK1 activation and the MAP kinase cascade (1-4). PD98059 binds to the inactive forms of MEK1 and prevents activation by upstream activators such as c-Raf (3)
Catalog #M2865-17A
SpecificityInhibits activation of MEK1 and MEK2 with IC50 values of 4uM and 50uM, respectively (1-3). Testing has shown that PD98059 does not inhibit activation of other highly related dual-specificity protein kinases or the activity of over 18 other Ser/Thr protein kinases (3). As shown in the Western Blot, at concentrations up to 100uM PD98059 does not inhibit activation of MKK3 or SEK (MKK4) as determined by measuring phosphorylation at its activation site. We have also found no inhibition of MKK6 or related family members.
ApplicationsSuitable for use in in vitro or cell-based assays. Other applications not tested.
Recommended DilutionMay be furhter diluted into aqueous buffers to yield desired concentrations
(< 100uM) with final DMSO concentrations of ~0.1%. IC50 values for inhibitory activity against MEK1 are around 510uM. For experiments with cultured cells, pretreat with M2865-17A for one hour prior to stimulation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with DMSO. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
CAS Number167869-21-8
Molecular FormulaC16H13NO3
Molecular Weight267.28
FormSupplied as a yellow, lyophilized powder. Reconstitution:
20mM stock1.5mg/280ul DMSO or 50mM stock: 1.5mg/112ul DMSO.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names2-(2-amino-3-methoxyphenyl)-4H-1-Benzopyran-4-one; 2-(2'-Amino-3'-methoxy)-flavone; 2-(2-amino-3-methoxyphenyl)-chromone


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