USBio Logo

M2865-17A MEK1 Inhibitor (PD98059, 2’-amino-3’-methoxyflavone) CAS: 167869-21-8

Specifications
References
CAS Number
167869-21-8
Grade
Highly Purified
Applications
WB
Molecular Formula
C16H13NO3
Molecular Weight
267.28
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C
2-(2-amino-3-methoxyphenyl)-4H-1-Benzopyran-4-one; 2-(2'-Amino-3'-methoxy)-flavone; 2-(2-amino-3-methoxyphenyl)-chromone

PD98059 (MEK1 Inhibitor) has been shown to act in vivo as a highly selective inhibitor of MEK1 activation and the MAP kinase cascade (1-4). PD98059 binds to the inactive forms of MEK1 and prevents activation by upstream activators such as c-Raf (3)

Specificity
Inhibits activation of MEK1 and MEK2 with IC50 values of 4uM and 50uM, respectively (1-3). Testing has shown that PD98059 does not inhibit activation of other highly related dual-specificity protein kinases or the activity of over 18 other Ser/Thr protein kinases (3). As shown in the Western Blot, at concentrations up to 100uM PD98059 does not inhibit activation of MKK3 or SEK (MKK4) as determined by measuring phosphorylation at its activation site. We have also found no inhibition of MKK6 or related family members.
Solubility
Soluble in anhydrous DMSO at 25mg/ml. Very poorly soluble in water.
Applications
Suitable for use in in vitro or cell-based assays. Other applications not tested.
Recommended Dilutions
May be further diluted into aqueous buffers to yield desired concentrations (<100uM) with final DMSO concentrations of ~0.1%. IC50 values for inhibitory activity against MEK1 are around 5-10uM. For experiments with cultured cells, pretreat with M2865-17A for one hour prior to stimulation. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder is stable for 6 months after receipt at -20°C. Reconstitute with DMSO. Stable for 3 months Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity
Highly Purified (~99%)
Concentration
Not Determined
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Dudley, D.T., et al., Proc. Natl. Acad. Sci. USA 92: 7686-7689 (1995). 2. Pang, L., et al., J. Biol. Chem. 270: 13,585-13,588 (1995). 3. Alessi, D.R., et al., J. Biol. Chem. 270: 27,489-27,494 (1995). 4. Lazar, D.F., et al., J. Biol. Chem. 270: 20,801-20,807 (1995). 5. Runden, E., et al., J. Neurosci. 18: 7296-7305 (1998). 6. Veeranna, et al., J. Neurosci. 18: 4008-4021 (1998). 7. Xing, J., et al., Mol. Cell Biol. 18: 1946-1955 (1998).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved