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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Merosin (Laminin alpha 2)

Anti -Merosin (Laminin alpha 2)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IP IH
Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. The LAMA2 gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy.
Catalog #M2990-02
Applications
Suitable for use in ELISA, Immunoprecipitation and Immunohistochemistry. Not recommended for Western Blot. Other applications have not been tested.
Recommended Dilutions:
ELISA: 0.02-0.1ug/ml
Immunoprecipitation: 1-10ug/ml in PBS.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D580
HostRat
SourceMouse
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 0.09% sodium azide, PEG and sucrose. Reconstitute with 1ml sterile ddH2O (15 minutes, RT).
PurityPurified by subsequent thiophilic adsorption and size exclusion chromatography.
ImmunogenMouse heart laminin-2 (merosin).
SpecificityRecognizes mouse laminin alpha2 at 300kD. Recognizes an N-terminal portion of the alpha2 chain that is deleted in congenital muscular dystrophies. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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