| Elements of the extracellular matrix such as laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in organs including the kidney, lung, skin, and nervous system. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. For example, the alpha subfamily of laminin chains is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. At least 15 distinct laminin trimers, containing various combinations of 5 alpha, 4 beta, and 3 gamma subunits have been found in mammals. Both laminin alpha 5 and alpha 1 are prevalent in kidney. Mice that are homozygous for a null mutation in the alpha 5 laminin gene are dead by embrionic day 14-19 with multiple developmental abnormalities. |
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| Applications: |
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| Suitable for use in ELISA, Immunohistochemistry, Western Blot, Immunofluorescence, Immunoaffinity Purification and |
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| Immunoprecipitation. Other applications not tested. |
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| Catalog # | M2990-50 |
| Recommended Dilutions | ELISA: 50% maximal binding to human merosin at 1:50,000. |
| Immunohistochemistry: 1:5000 for staining of 8um acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody. |
| Western Blot: 1:1000 detected Merosin on 10ug of human placental lysate. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| Control | Human placenta lysate, vascular breast carcinomas, cultured embryonic retinal neurons. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q598 |
| Host | Mouse |
| Source | Human |
| Concentration | Not Determined |
| Form | Supplied as a liquid in buffer. No preservative added. |
| Purity | Ascites |
| Immunogen | Purified human merosin. |
| Specificity | Recognizes the 80kD fragment of the M-chain of human merosin. Species Crossreactivity: monkey and rabbit. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
