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You are here:Home » Culture Media » Media-Cell Culture » Mesenchymal Stem Cells, Mouse, Freezing Medium

Mesenchymal Stem Cells, Mouse, Freezing Medium


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Catalog #M3005-34C
DescriptionMouse MSC freezing medium was optimized for cryopreservation of our Mouse mesenchymal stem cells (mMSC).
This product contains fetal bovine serum. Each lot is tested for sterility.
Procedure for Freezing mMSC1. Freeze mouse MSC cells when they reach > 95% confluence.
2. Warm Dulbecco’s PBS (without Ca2+ and Mg2+ bath. Thaw mMSC freezing medium at room temperature.) and 0.05% trypspin/EDTA in a 37°C water
3. Remove mMSC growth medium, rinse mMSC with Dulbecco’s PBS, and incubate the cells with trypsin/EDTA solution until approximately 90% of the cells begin to detach. Monitor the cells under a microscope to avoid over-trypsinization.
4. Add fetal bovine serum equal to 1/10 volume of the trypsin/EDTA solution to neutralize trypsin. Gently shake the culture vessel to mix.
5. Transfer the cell suspension into a 15ml conical tube. Take a small aliquot of the suspension to count cells. Centrifuge the cell suspension 250 x g for 5 minutes at room temperature.
6. Carefully remove the supernatant. Add a volume of mMSC freezing medium and resuspend cells to 1 x 10e6 per ml. Dispense 1ml into each cryovial.
7. Place cryovials in a freezing container to slowly lower the temperature in a -80°C freezer. Transfer the vials to liquid nitrogen the next day for long term storage.
Storage and StabilityStore at -20°C. Stable for 1 year from the date of receipt under proper storage condition.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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