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Anti -c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
Anti -c-Met, CT (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IH |
|
| Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor. The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain. Both chains are derived from a single precursor protein by proteolytic cleavage. c-Met is expressed in a wide variety of adult and embryonic tissues. It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF. Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas. c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis. Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53 (1). | | | Catalog # | M3007-13 | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | ELISA (Immunogen): 0.1-1ug/ml | | Western Blot: 1-2ug/ml | | Immunohistochemistry (Paraffin): 1-2ug/ml. Best results obtained by subjecting tissue to heat-induced epitope retrieval (HIER). | | Optimal dilutions to be determined by the researcher. | | Positive Controls | Western Blot: A431 cell extracts | | Immunohistochemistry: Human breast cancer tissue | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.25mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. Available without azide, BSA and glycerol. See M3007-13A. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein. | | Specificity | Recognizes the human c-Met protein at ~145kD. Does not crossreact with related proteins. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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