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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Met, phosphorylated (Tyr1234/1235) (Agarose)

Anti -Met, phosphorylated (Tyr1234/1235) (Agarose)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant IP
Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45kD alpha and 145 kD beta-subunits (1,2). The alpha-subunit and the amino-terminal region of the beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in auto-phosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6).
Catalog #M3007-22C
ApplicationsSuitable for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunoprecipitation: Add 10ul of M3007-22C (well-vortexed) to 200ul of cell lysate at 1mg/ml in 1X Cell Lysis Buffer (20mM Tris, pH 7.5, 150mM sodium chloride, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 2.5mM sodium pyrophosphate, 1mM beta-glycerophosphate, 1mM Na3VO4, 1ug/ml Leupeptin, 1mM PMSF.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No10E38
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid suspension in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Coupled to Agarose.
PuritySupernatant
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding Tyr1234/1235 of human Met.
SpecificityRecognizes endogenous levels of human Met at ~145kD only when phosphorylated at Tyr1234/1235. May crossreact with overexpressed tyrosine phosphorylated Src proteins. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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