Anti -Met, phosphorylated (Tyr1234/1235) (Agarose)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
IP |
|
| Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45kD alpha and 145 kD beta-subunits (1,2). The alpha-subunit and the amino-terminal region of the beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in auto-phosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon and breast. Thus, Met is an attractive cancer therapeutic and diagnostic target (6). | | | Catalog # | M3007-22C | | Applications | Suitable for use in Immunoprecipitation. Other applications not tested. | | Recommended Dilutions | Immunoprecipitation: Add 10ul of M3007-22C (well-vortexed) to 200ul of cell lysate at 1mg/ml in 1X Cell Lysis Buffer (20mM Tris, pH 7.5, 150mM sodium chloride, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 2.5mM sodium pyrophosphate, 1mM beta-glycerophosphate, 1mM Na3VO4, 1ug/ml Leupeptin, 1mM PMSF. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 10E38 | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid suspension in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Coupled to Agarose. | | Purity | Supernatant | | Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr1234/1235 of human Met. | | Specificity | Recognizes endogenous levels of human Met at ~145kD only when phosphorylated at Tyr1234/1235. May crossreact with overexpressed tyrosine phosphorylated Src proteins. Species Crossreactivity: mouse and rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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