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Anti -Methionine Aminopeptidase 2 (MetAP-2)
Anti -Methionine Aminopeptidase 2 (MetAP-2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B IP |
|
| In vitro, the metalloprotease MetAP-2 catalyzes the hydrolysis of N-terminal methionine residues. MetAP-2 is highly conserved across species. The catalytic domains of S. cerevisiae and human MetAP-2 proteins are ~80% homologous. The function of MetAP-2 in vivo remains to be determined. MetAP-2 has been identified, purified and cloned from rat cells as a 67kD eIF-2-associated (elongation inhibitory factor-2) protein, suggesting a possible role in protein translation. Provocative experiments suggest MetAP-2 may also be involved in angiogenesis, the process of new blood vessel formation. In these studies, MetAP-2 was determined to be the major cellular protein covalently bound to the potent anti-angiogenic agent fumagillin, following fumagillin treatment of HUVEC (human umbilical endothelial cells) cells or calf brain homogenates. Fumagillin was able to selectively inhibit the growth of a MetAP-2-dependent yeast strain. The fungal derived fumagillin, and its more potent metabolite TNP-470 (now in clinical trials), are effective at limiting both the size and metastasis of solid tumors. TNP-470 inhibits neovascularization via endothelial cell cycle arrest in the late G1 phase. The details of this process are not well understood. In view of the identification of MetAP-2 as a fumagillin binding protein, future studies with MetAP-2 should provide important new insights into the mechanisms of fumagillin/TNP- 470 induced cell-cycle arrest and the role of MetAP-2 in angiogenesis. | | | Catalog # | M3016-01 | | Applications | Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | ELISA: 0.5-1ug/ml | | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Sf 21-derived, recombinant human Methionine Aminopeptidase 2 (aa2-478). | | Specificity | Recognizes human METAP2. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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