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You are here:Home » Antibodies » Abs to Amino Acids » Anti -Methionine Aminopeptidase 2 (MetAP-2)

Anti -Methionine Aminopeptidase 2 (MetAP-2)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IP
In vitro, the metalloprotease MetAP-2 catalyzes the hydrolysis of N-terminal methionine residues. MetAP-2 is highly conserved across species. The catalytic domains of S. cerevisiae and human MetAP-2 proteins are ~80% homologous. The function of MetAP-2 in vivo remains to be determined. MetAP-2 has been identified, purified and cloned from rat cells as a 67kD eIF-2-associated (elongation inhibitory factor-2) protein, suggesting a possible role in protein translation. Provocative experiments suggest MetAP-2 may also be involved in angiogenesis, the process of new blood vessel formation. In these studies, MetAP-2 was determined to be the major cellular protein covalently bound to the potent anti-angiogenic agent fumagillin, following fumagillin treatment of HUVEC (human umbilical endothelial cells) cells or calf brain homogenates. Fumagillin was able to selectively inhibit the growth of a MetAP-2-dependent yeast strain. The fungal derived fumagillin, and its more potent metabolite TNP-470 (now in clinical trials), are effective at limiting both the size and metastasis of solid tumors. TNP-470 inhibits neovascularization via endothelial cell cycle arrest in the late G1 phase. The details of this process are not well understood. In view of the identification of MetAP-2 as a fumagillin binding protein, future studies with MetAP-2 should provide important new insights into the mechanisms of fumagillin/TNP- 470 induced cell-cycle arrest and the role of MetAP-2 in angiogenesis.
Catalog #M3016-01
ApplicationsSuitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSf 21-derived, recombinant human Methionine Aminopeptidase 2 (aa2-478).
SpecificityRecognizes human METAP2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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