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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -5-Methoxytryptamine, Conjugated

Anti -5-Methoxytryptamine, Conjugated


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Clone Host Grade Applications
Polyclonal Rabbit Purified E IH
Catalog #M3300
Applications Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Specificity Using a conjugate 5 Methoxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds
Compounds Cross-reactivity ratio(a) 5 Methoxytryptamine-G-PL 1 5 Methoxytryptamine-G-PL (b) 1/12 5 Methoxytryptamine 1/22 5 Hydroxytryptamine-G-PL 1/26 Tryptamine-G-PL 1/30 5 Methoxytryptophan-G-PL 1/40 Tryptophan 1/495 5 Hydroxytryptophan-G-PL 1/1,902
(a) 5 Methoxytryptamine-G-PL concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
(b) : Non-reduced conjugate
G = Glutaraldehyde, PL = poly-Lysine
Recommended dilution The antiserum was tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 5-methoxytryptamine antibodies stains well between a 1/2,000-1/5,000 dilution.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Immunogen5 Methoxytryptamine Glutaraldehyde Carriers.
SpecificityRecognizes protein-conjugated 5-Methoxytryptamine. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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