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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -5-Methoxytryptophan, Conjugated

Anti -5-Methoxytryptophan, Conjugated


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Clone Host Grade Applications
Polyclonal Rabbit Purified E
Catalog #M3350
Applications Suitable for use in ELISA. Other applications not tested.
Specificity Using a conjugate 5-methoxytryptophan-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds
Compounds Cross-reactivity ratio(a) 5-Methoxytryptophan-G-BSA 1 5-Hydroxytryptophan-G-BSA 1/>100,000 Tryptophan-G-BSA 1/5800 5-Methoxytryptamine-G-BSA 1/100,000
(a) 5-Methoxytryptophan-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin
Recommended dilution The anti-conjugated 5-methoxytryptophan antibodies stains well between a 1/2,000-1/5,000 dilution.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilizaed powder in PBS, pH 7.2. No preservative added.
PurityAntiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
SpecificityRecognizes protein conjugated 5-Methoxytryptophan. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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