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You are here:Home » Antibodies » Abs to Nucleotides » Anti -5-Methylcytidine

Anti -5-Methylcytidine

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH FC IF
Catalog #M3398-55M
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Immunoblot, Methylated DNA Immunoprecipitation, and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended DilutionFlow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
HybridomaSp2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B11
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.01% thimerosal.
PurityPurified
Immunogen5-Methylcytidine.
SpecificityRecognizes the modified base 5-methylcytidine (5-MeCyd) found in DNA of plants and vertebrates. Species Crossreactivity: human, rat, mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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