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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Mindbomb homolog 1 (MIB1) is an E3 ligase that facilitates the ubiquitination and the subsequent endocytosis of the Notch ligands, Delta and Jagged (1,2). MIB1 appears to promote the ubiquitination and degradation of death-associated protein kinase (DAPK1) in vitro (3). Expression of MIB1 is seen in both adult and embryonic murine tissues (4). Recently, MIB1 was reported to regulate the extrinsic cell death pathway by binding to cellular FLICE-like inhibitory proteins (cFLIP-L and cFLIP-S), which reduces the interaction of caspase-8 with cFLIP and leads to cell death (5). MIB1 is also involved in T and marginal zone B (MZB) cell development in the lymphopoietic niches (6).
Catalog #	M3753
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
	Tween-20 at 4°C with gentle shaking, overnight.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of human MIB1 protein (KLH-coupled).
Specificity	Recognizes human MIB1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.