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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -MIB1 (E3 Ubiquitin-protein Ligase MIB1, Mind Bomb Homolog 1, DAPK-interacting Protein 1, DIP-1, Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2, MIB1, DIP1, KIAA1323, ZZANK2)

Anti -MIB1 (E3 Ubiquitin-protein Ligase MIB1, Mind Bomb Homolog 1, DAPK-interacting
Protein 1, DIP-1, Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2, MIB1, DIP1,
KIAA1323, ZZANK2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
MIB1 (Mind bomb 1 also DIP1 and ZZANK2) is a 110-115kD E3 ubiquitin ligase. It is widely expressed in both embryo and adult, and appears to have a non redundant role in Notch receptor signaling. Notch signaling is initiated when a transmembrane (TM) Notch ligand (Jagged or Delta) binds to Notch on an adjacent cell. This ligation induces cleavage of the Notch extracellular domain (ECD) with the creation of a Jagged/Delta:NotchECD complex. This complex is now internalized by the Notch ligand expressing cell. The displacement of the NotchECD from its TM segment promotes further Notch processing, resulting in Notch signaling. The key internalization step by the ligand expressing cell is mediated by the MIB1 E3 ubiquitin ligase. Human MIB1 is 1006aa in length. It contains multiple interspersed Ankyrin repeats, MIB domains, and zinc finger motifs. There is one potential alternative start site at Met84. Over aa365-461, human MIB1 shares 99% aa sequence identity with mouse MIB1.
Catalog #M3753-01C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa365-461 from human MIB1 expressed in E.coli.
SpecificityRecognizes human MIB1. Species Crossreactivity: mouse and rat, recombinant MIB2 (<1%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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