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You are here:Home » Culture Media » Media-Cell Culture, MEM Eagle » MEM Eagle w/Earle’s, L-Glutamine w/o Leucine, Lysine, Methionine (Powder)

MEM Eagle w/Earle’s, L-Glutamine w/o Leucine, Lysine, Methionine (Powder)

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Specifications

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.  Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.
Catalog #M3875
Appearance
White to off white, homogenous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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