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You are here:Home » Antibodies » Abs to Receptors, Estrogen » Anti -GRIN2D (Glutamate receptor, ionotropic, N-methyl D-aspartate 2D, EB11, NMDAR2D, Estrogen receptor binding CpG island)

Anti -GRIN2D (Glutamate receptor, ionotropic, N-methyl D-aspartate 2D, EB11, NMDAR2D,
Estrogen receptor binding CpG island)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E
Catalog #M3884-81H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionPeptide ELISA: 1:32,000.
Western Blot: 0.02ug/ml. A band is detected at ~80kD in Human Brain (Cerebellum) and in Mouse Brain and Rat Brain lysates. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See M3884-81H-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide from the C-terminus of aa-TRRGSAHFSSLESE
SpecificityRecognizes human GRIN2D. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesGRIN2D; glutamate receptor, ionotropic, N-methyl D-aspartate 2D; EB11; NMDAR2D; N-methyl-D-aspartate receptor subunit 2D; estrogen receptor binding CpG island


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