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Abs to Enzymes, Methyltransferase
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Anti -MGMT (O-6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA Methyltransferase, O-6-Methylguanine DNA Alkyltransferase)
Anti -MGMT (O-6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA
Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA
Methyltransferase, O-6-Methylguanine DNA Alkyltransferase)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IH FC |
|
| Cancer chemotherapeutic alkylating agents (e.g. BCNU,) induce the formation of lethal cross-links at the O6-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer process inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA- damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug-resistant. | | | Catalog # | M3884-90D | | Applications | Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-1ug/ml for 2h, RT | | Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 for 30min at RT | | Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10min followed by cooling at RT for 20min | | Optimal dilutions to be determined by the researcher. | | Positive Control | Raji cells. Colon carcinoma | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | SPM287 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant corresponding to human MGMT. | | Specificity | Recognizes human MGMT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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