| O6-Methylguanine-DNA Methyltransferase (MGMT) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine, primarily O6 methylguanine lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. The mechanism of MGMT action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. MGMT is frequently classified as a suicide protein. The repair capacity for O6- methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents. |
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| Catalog # | M3884-91 |
| Applications | Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:500 detects MGMT on 10ug of Jurkat lysates. |
| Immunohistochemistry: Paraffin sections |
| Immunofluorescence (IC): HeLa cells |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Tonsil tissue, HeLa cells, CEM-CCRF cells. |
| Negative Control | TK6 cells |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 2Q434 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography |
| Immunogen | Recombinant protein corresponding to human MGMT (O6-methylguanine-DNA methyltransferase) expressed in E. coli. |
| Specificity | Recognizes MGMT in human tissues, tumors, cells or xenograft lines at ~22-25kD. Gives weak cross-reactivity with MGMT from mouse, hampster and rat cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
