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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -MGMT (O6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA Methyltransferase, O6 Methylguanine DNA Alkyltransferase)

Anti -MGMT (O6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA
Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA
Methyltransferase, O6 Methylguanine DNA Alkyltransferase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH FC
O6-Methylguanine-DNA-Methyltransferase (MGMT) is a 22kD human DNA repair protein that removes O6-alkylguanine DNA adducts. MGMT acts as a suicide acceptor protein repairing the DNA by accepting alkyl groups and consequently inactivating itself. This antibody stains all mantle zone lymphocytes and 50%of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody.
Catalog #M3884-91G
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: Predicted MW 21.5kD. 25kD band is seen.
Immunohistochemistry: Staining Protocol incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections with 10 mM citrate buffer, pH 6.0 must be performed prior to the immunostaining.
Optimal dilutions to be determined by the researcher.
Cellular Localization Nuclear and cytoplasmic.
Positive Control Tonsil
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L758
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM PBS, pH7.4, 0.2% BSA.
PurityTissue culture supernatant
ImmunogenRecombinant human MGMT protein.
SpecificityStains all mantle zone lymphocytes and 50% of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody. Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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