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Abs to Enzymes, Methyltransferase
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Anti -MGMT (O6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA Methyltransferase, O6 Methylguanine DNA Alkyltransferase)
Anti -MGMT (O6-Methylguanine DNA Methyltransferase, 6-O-Methylguanine DNA
Methyltransferase, Methylated DNA Protein Cysteine Methyltransferase, Methylguanine DNA
Methyltransferase, O6 Methylguanine DNA Alkyltransferase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Supernatant |
B IH FC |
|
| O6-Methylguanine-DNA-Methyltransferase (MGMT) is a 22kD human DNA repair protein that removes O6-alkylguanine DNA adducts. MGMT acts as a suicide acceptor protein repairing the DNA by accepting alkyl groups and consequently inactivating itself. This antibody stains all mantle zone lymphocytes and 50%of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody. | | | Catalog # | M3884-91G | | Applications | Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: Predicted MW 21.5kD. 25kD band is seen. | | Immunohistochemistry: Staining Protocol incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections with 10 mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. | | Optimal dilutions to be determined by the researcher. | | Cellular Localization | Nuclear and cytoplasmic. | | Positive Control | Tonsil | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8L758 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM PBS, pH7.4, 0.2% BSA. | | Purity | Tissue culture supernatant | | Immunogen | Recombinant human MGMT protein. | | Specificity | Stains all mantle zone lymphocytes and 50% of germinal center lymphocytes. Basaloid epithelial cells of tonsil squamous mucosa were also stained positive with this antibody. Species Crossreactivity: Human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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