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Anti -MGMT (O6-Methylguanine DNA Methyltransferase)
Anti -MGMT (O6-Methylguanine DNA Methyltransferase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
IH |
|
| O6-methylguanine-DNA methyltransferase (MGMT) is a ubiquitous 22kD DNA repair protein, involved in the repair of mutagenic, carcinogenic, and cytotoxic O6-alkylguanine adducts, including primary alkyl adducts, induced by the chloroethylating and methylating agents used in the treatment of cancers. MGMT transfers alkyl groups from the O6 position of guanine in DNA to a cysteine residue in its sequence, restoring the integrity of DNA. This transfer is irreversible and inactivates MGMT; a cell’s ability to withstand damage is directly related to the number of MGMT molecules within the cell and the rate of MGMT synthesis. MGMT repair thus represents a major form of chemotherapeutic drug resistance. The importance of MGMT in determining clinical response, both to chloroethylnitrosourea chemotherapeutic agents and to carcinogenesis by methylating agents, has been described. MGMT expression is highly variable in normal and tumor tissues, and also varies between individual cancer cells within a tumor. | | | Catalog # | M3884-92B | | Applications | Suitable for use in Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry (FFPE): Heat Induced Epitope Retrival (HIER) required (EDTA pH 8.0.). Enzyme digestion not required. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Breast cancer/lung cancer/ovarian cancer tissue sections | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 6A129 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, and 1% BSA, 0.1% sodium azide. | | Purity | Purified from mouse ascites | | Immunogen | Recombinant human MGMT. Expected Staining Pattern: Nuclear | | Specificity | Recognizes human MGMT | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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