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You are here:Home » Antibodies » Antibodies-Enzymes, Methyltransferase » Anti -MGMT (O6-Methylguanine DNA Methyltransferase)

Anti -MGMT (O6-Methylguanine DNA Methyltransferase)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
O6-methylguanine-DNA transferase (MGMT) is a ubiquitous 22kD DNA repair protein, involved in the repair of mutagenic, carcinogenic, and cytotoxic O6-alkylguanine adducts, including primary alkyl adducts, induced by the chloroethylating and methylating agents used in the treatment of cancers. MGMT transfers alkyl groups from the O6 position of guanine in DNA to a cysteine residue in its sequence, restoring the integrity of DNA. This transfer is irreversible and inactivates MGMT; a cellís ability to withstand damage is directly related to the number
of MGMT molecules within the cell and the rate of MGMT synthesis. MGMT repair thus represents a major form of chemotherapeutic drug resistance. The importance of MGMT in determining clinical response, both to chloroethylnitrosourea chemotherapeutic agents and to carcinogenesis by methylating agents, has been described. MGMT expression is highly variable in normal tissues as well as in cancer cells.10 A fraction of primary tumor cells and ~20% of human tumor cell lines lack MGMT expression. Hypermethylation of the Mgmt promoter region has been linked to loss of MGMT protein expression in cancer cell lines and tissues. Tumor cell lines lacking MGMT are highly sensitive to alkylating agents and nitrosourea-type agents, suggesting that selective inhibition of MGMT in tumor cells may be an effective strategy in overcoming chemotherapeutic drug resistance.
Catalog #M3884-92C
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and Flow Cytometry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Flow Cytometry: 5-10ug/ml
Immunohistochemistry: 10ug/ml
Note: Staining results for immunohistochemical assays with formalin-fixed, paraffin-embedded tissue sections may be greatly enhanced with an antigen retrieval pre-treatment step using citrate buffer, pH 6.0.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6A129
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A chromatography.
ImmunogenRecombinant human MGMT protein purified from E. coli.
SpecificityRecognizes the human MGMT protein. Reactivity is confirmed with human T cell leukemia CEM/CCRF cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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