| O6-methylguanine-DNA transferase (MGMT) is a ubiquitous 22kD DNA repair protein, involved in the repair of mutagenic, carcinogenic, and cytotoxic O6-alkylguanine adducts, including primary alkyl adducts, induced by the chloroethylating and methylating agents used in the treatment of cancers. MGMT transfers alkyl groups from the O6 position of guanine in DNA to a cysteine residue in its sequence, restoring the integrity of DNA. This transfer is irreversible and inactivates MGMT; a cell’s ability to withstand damage is directly related to the number |
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| of MGMT molecules within the cell and the rate of MGMT synthesis. MGMT repair thus represents a major form of chemotherapeutic drug resistance. The importance of MGMT in determining clinical response, both to chloroethylnitrosourea chemotherapeutic agents and to carcinogenesis by methylating agents, has been described. MGMT expression is highly variable in normal tissues as well as in cancer cells.10 A fraction of primary tumor cells and ~20% of human tumor cell lines lack MGMT expression. Hypermethylation of the Mgmt promoter region has been linked to loss of MGMT protein expression in cancer cell lines and tissues. Tumor cell lines lacking MGMT are highly sensitive to alkylating agents and nitrosourea-type agents, suggesting that selective inhibition of MGMT in tumor cells may be an effective strategy in overcoming chemotherapeutic drug resistance. |
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| Catalog # | M3884-92C |
| Applications | Suitable for use in Immunohistochemistry, Western Blot and Flow Cytometry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1-3ug/ml |
| Flow Cytometry: 5-10ug/ml |
| Immunohistochemistry: 10ug/ml |
| Note: Staining results for immunohistochemical assays with formalin-fixed, paraffin-embedded tissue sections may be greatly enhanced with an antigen retrieval pre-treatment step using citrate buffer, pH 6.0. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 6A129 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A chromatography. |
| Immunogen | Recombinant human MGMT protein purified from E. coli. |
| Specificity | Recognizes the human MGMT protein. Reactivity is confirmed with human T cell leukemia CEM/CCRF cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
