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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IP IH FC

Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr ~44kD) non-covalently bound to the invariant beta2-microglobulin subunit (Mr ~12kD). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.1-3
Catalog #	M3885-48A
Applications	• Flow cytometry 1-3
	• Immunoprecipitation 1-3
	• Western blotting
	• Immunohistochemistry
	• Immunoaffinity chromatography
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5K89
Host	Mouse
Source	Chicken
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
Purity	Purified
Specificity	Recognizes a monomorphic epitope on avian MHC Class I molecules (Mr ~68kD). Also reacts with turkey MHC Class I.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.