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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1 (PE)

Anti -MHC Class 1 (PE)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IP IH FC
Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr ~44kD) non-covalently bound to the invariant beta2-microglobulin subunit (Mr ~12kD). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.1-3
Catalog #M3885-48C
Applications Flow cytometry 1-3
Immunoprecipitation 1-3
Western blotting
Immunohistochemistry
Immunoaffinity chromatography
Recommended DilutionFlow Cytometry: 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K89
HostMouse
SourceChicken
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
PurityPurified
SpecificityRecognizes a monomorphic epitope on avian MHC Class I molecules (Mr ~68kD). Also reacts with turkey MHC Class I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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