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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1 (Biotin)

Anti -MHC Class 1 (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH FC
Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.
Catalog #M3885-48E
ApplicationsSuitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Functional Application: Antibody (whole molecule) is cytotoxic in the presence of complement (1). Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Flow Cytometry Analysis5x10e5 Ficoll prepared peripheral blood mononuclear cells (PBMC) were washed and incubated 45 minutes on ice with 80ul of M3885-48E at 5ug/ml. Cells were washed twice and incubated with secondary reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.28 log10 fluorescent units when compared to a mouse IgG2a/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5 mg/ml anti-MHC Class I antibody M3885-48D (unconjugated clone 7H121).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H121
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS containing 5% glycerol, 0.2% BSA, 0.04% sodium azide
PurityPurified by Protein A affinity chromatography
ImmunogenHuman HSB-2 T cell line
SpecificityRecognizes a monomorphic Class I epitope on the a chain
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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