Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH FC

Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.
Catalog #	M3885-48F
Applications	Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:50 (80ul/test) Antibody (whole molecule) is cytotoxic in the presence of complement.
	Optimal dilutions to be determined by the researcher.
Flourescein/IgG molar ratio	7.4
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H121
Host	Mouse
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM sodium chloride 5% Glycerol, 0.2% BSA, 0.04% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human HSB-2 T cell line.
Specificity	Recognizes a monomorphic Class I epitope on the a chain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.