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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified FC

Human MHC Class I (HLA-A, B & C) molecules are expressed on nearly all nucleated cells. MHC Class I molecules consist of heterodimers of a highly polymorphic transmembrane a chain and an invariant b chain. MHC Class I molecules bind to endogenously synthesized peptides of about 8-10 amino acids which are presented to CD8+ T cells. Recent evidence now indicates exogenously derived peptides are also presented by MHC Class I molecules.
Catalog #	M3885-48G
Applications	Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution;Flow Cytometry	1:50 (or use 1.6ul concentrated stock per 5x10e5 cells per test)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H121
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS containing 15% glycerol, 0.2% BSA, and 0.04% sodium azide. Labeled with R-Phycoerythrin.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human HSB-2 T cell line
Specificity	Recognizes human MCH Class I (HLA-A, B and C) molecules. Recoognizes a monomorphic Class I epitope on the alpha chain.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.