| Catalog # | M3885-49H |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Flow Cytometry (rat splenocytes): 1:50-1:100, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. |
| Immunohistochemistry (frozen): Acetone fixation recommended, the antigen is sensitive to fixation with paraformaldehyde. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | Goat anti-mouse IgG: FITC (rat absorbed), IgG Goat anti mouse IgG: RPE (rat adsorbed) |
| Recommended Negative Controls | Mouse IgG1 Negative Control |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2655 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Rat spleen cell glycoprotein. |
| Specificity | Recognizes a monomorphic determinant of rat MHC Class I |
| (RT-1A), expressed by all rat strains. Quantitative measurements suggest that not all of the class I molecules are recognized. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
