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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1, RT 1a (FITC)

Anti -MHC Class 1, RT 1a (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Catalog #M3885-49L
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Recommended Negative Controls Mouse IgG1 Negative Control: FITC
ApplicationsFlow Cytometry: Neat-1:10
Immunohistochemistry (frozen)
Optimal working dilutions to be determined by researcher.
Flow Cytometry Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: Acetone fixation recommended-the antigen is sensitive to fixation with paraformaldehyde.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2655
HostMouse
SourceRat
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenRat spleen cell glycoprotein.
SpecificityThis antibody labels a monomorphic determinant of rat MHC Class I (RT-1A), however quantitative measurements suggest that not all of the Class I molecules are recognized. The antibody has been used in immunoaffinity purification of rat MHC Class I molecules.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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