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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC

Catalog #	M3885-49L
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
Recommended Negative Controls	Mouse IgG1 Negative Control: FITC
Applications	Flow Cytometry: Neat-1:10
	Immunohistochemistry (frozen)
	Optimal working dilutions to be determined by researcher.
Flow Cytometry	Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
	Immunohistochemistry: Acetone fixation recommended-the antigen is sensitive to fixation with paraformaldehyde.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H2655
Host	Mouse
Source	Rat
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified by Protein G affinity chromatography.
Immunogen	Rat spleen cell glycoprotein.
Specificity	This antibody labels a monomorphic determinant of rat MHC Class I (RT-1A), however quantitative measurements suggest that not all of the Class I molecules are recognized. The antibody has been used in immunoaffinity purification of rat MHC Class I molecules.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.