Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH FC

MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin.3, 4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3, 6 This antibody binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin.7,8
Catalog #	M3885-59A
Applications	• Flow cytometry
	• Immunohistochemistry (frozen tissue sections)
	• Complement-mediated cytotoxicity
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	28-14-8
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity	Purified
Immunogen	C3H.SW mouse splenocytes
Specificity	Recognizes an epitope in the alpha3 domain of H-2Db. It crossreacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.