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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1, H2, Db, alpha3 (Biotin)

Anti -MHC Class 1, H2, Db, alpha3 (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP FC
MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin (3,4). These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells (5). MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny (3,6). This antibody binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin (7,8).
Catalog #M3885-59C
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Complement-mediated cytotoxicity studies. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No28-14-8
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.
PurityPurified
ImmunogenC3H.SW mouse splenocytes
SpecificityRecognizes an epitope in the alpha3 domain of mouse H-2Db. Crossreacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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