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Anti -MHC Class 1, H2, Db, alpha3 (Biotin)
Anti -MHC Class 1, H2, Db, alpha3 (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
IP FC |
|
| MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin (3,4). These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells (5). MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny (3,6). This antibody binds to the alpha3 domain of H-2Db in the presence or absence of beta2 microglobulin (7,8). | | | Catalog # | M3885-59C | | Applications | Suitable for use in Flow Cytometry, Immunoprecipitation and Complement-mediated cytotoxicity studies. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 1ug/10e6 cells | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 28-14-8 | | Host | Mouse | | Source | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin. | | Purity | Purified | | Immunogen | C3H.SW mouse splenocytes | | Specificity | Recognizes an epitope in the alpha3 domain of mouse H-2Db. Crossreacts with the alpha3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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