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You are here:Home » Antibodies » Abs to MHC » Anti -MHC class I, H-2Ld, Db (H-2Ld/H-2Db)

Anti -MHC class I, H-2Ld, Db (H-2Ld/H-2Db)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
This antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody has been reported to block antigen-specific lysis of target cells by cytotoxic T lymphocytes. This antibody cross-reacts with the H-2Ld haplotype. This clone recognizes the a3 domain of H-2Db.
Catalog #M3885-59R
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 0.25ug/10e6 cells in 100ul volume
Immunohistochemistry: Acetone-fixed frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by affinity chromatography
ImmunogenC3H.SW mouse splenocytes
SpecificityRecognizes mouse b and d haplotype. Crossreactivity: H-2Ld. Does not react with other haplotypes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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