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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP FC

This antibody reacts with the H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. H-2Dk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Reactivity with other haplotypes (e.g., a,b,p,q,r,s) has not been reported.
Catalog #	M3885-60K
Applications	Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells in 100ul volume
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	C3H mouse splenocytes
Specificity	Recognizes H-2Dk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Dk haplotype. Weakly cross-reacts with H-2Kd and with cells from mice of the H-2f haplotype. Does not cross react with other haplotypes (e.g., a, b, p, q, r, s).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.