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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1, H2, Dd

Anti -MHC Class 1, H2, Dd

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP FC
This antibody has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
Catalog #M3885-63
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation, Immunoaffinity chromatography and Antigen blocking. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No5K47
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
ImmunogenC57BL/6 x DBA/2)F1 hybrid mouse splenocytes
SpecificityReacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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