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You are here:Home » Antibodies » Antibodies-MHC » Anti -MHC Class 1, H2, Dd (FITC)

Anti -MHC Class 1, H2, Dd (FITC)


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Clone Host Grade Applications
Monoclonal Mouse Purified IP FC
Reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
Catalog #M3885-63A
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation, Blocking of antigen recognition and Complement-dependent cytotoxicity. Other applications not tested.
Recommended DilutionFlow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5K47
FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
ImmunogenC57BL/6 x DBA/2)F1 hybrid mouse splenocytes
SpecificityRecognizes a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro and cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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