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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP FC

This antibody has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
Catalog #	M3885-63C
Applications	• Flow cytometry1
	• Complement-dependent cytotoxicity1
	• Blocking of antigen recognition
	• Immunoprecipitation
	• Immunoaffinity chromatography
Recommended Dilution	Flow Cytometry: 0.2ug/10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	5K47
Host	Mouse
Source	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Conjugated to PE.
Purity	Purified
Immunogen	C57BL/6 x DBA/2)F1 hybrid mouse splenocytes
Specificity	Reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.