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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1, H2, Kd, alloantigen (H-2Kd)

Anti -MHC Class 1, H2, Kd, alloantigen (H-2Kd)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC
This antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. This antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). It recognizes the a3 domain of kd.
Catalog #M3885-72E
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone NoSF1-1.1
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenBALB/c mouse cells
SpecificityRecognizes the alpha3 domain of mouse H-2Kd MHC class I. Crossreactivity: H-2k haplotype (weakly). Does not cross react with other haplotypes (e.g., b, j, p, q, s, v).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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