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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 1, H2, Kd, Dd

Anti -MHC Class 1, H2, Kd, Dd

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH FC
MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6
Catalog #M3885-73R
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Complement-mediated cytotoxicity. Other applications not tested.
Recommended DilutionFlow Cytometry: 1ug/10e6 cells
Immunohistochemistry: acetone-fixed, frozen tissue sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No34-1-2S
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
PurityPurified
ImmunogenC3H.SW mouse splenocytes
SpecificityRecognizes an epitope in the alpha3 domain that is common to H-2Kd and H-2Dd. Binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of b2 microglobulin. It crossreacts with the alpha3 domain of H-2Kb.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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