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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH FC

Catalog #	M3885-85B
Applications	• Flow cytometry
	• Complement-dependent cytotoxicity
	• Immunohistochemistry (acetone-fixed, frozen sections only)
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	5K51
Host	Mouse
Source	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Purity	Purified
Immunogen	A.AL mouse splenocytes
Specificity	Reacts with the H-2Kk MHC class I alloantigen. Crossreactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. Does not react with other (e.g., b, d, q) haplotypes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.