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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2, I-Ab (MHC Class II, I-Ab)

Anti -MHC Class 2, I-Ab (MHC Class II, I-Ab)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
This antibody reacts with the I-Ab MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. This antibody cross-reacts with the I-Af, I-Ar, I-As, and I-Av haplotypes.
Catalog #M3886-08F
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.25ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenC57BL/10 mouse splenocytes
SpecificityRecognizes mouse b haplotype. Crossreactivity: I-A f,r,s,v haplotypes. Does not react with other haplotypes (e.g., a, d, k, n, p, q, u).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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