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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class 2 (HLA DP)

Anti -MHC Class 2 (HLA DP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kD) and beta (27-30kD) chains. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
Catalog #M3887-10
ApplicationsSuitable for use in Flow Cytometry, immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: acetone fixed frozen
Optimal dilutions to be determined by researcher.
Cellular LocalizationCell membrane
Positive ControlTonsil or lymph node
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No0.N.418
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and Azide. See M3887-10X.
PurityPurified by Protein A affinity chromatography.
ImmunogenNon-T, non-B human acute lymphoblastic leukemia REH6 cell line
SpecificityReacts with B- and non-T, non-B cell lines. Does not react with T- and myeloid cell lines and leukemias. Recognizes a non polymorphic determinant of DP-MHC class II. Species Crossreactivity: Human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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