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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH FC

During ontogeny the level of B-L expression is reported to increase during the bursal phase of B cell differentiation.
Catalog #	M3887-10B1
Fusion Partners	Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Applications	Flow Cytometry 1:50-1:100
	Immunohistochemistry: Frozen
	Immunoprecipitation
	Flow Cytometry: Use 10ul of the suggested working dilution to label 10 e6cells in 100ul.
Recommended Secondary Reagents	F(ab')2 Rabbit anti mouse IgG: FITC
	F(ab')2 Rabbit anti mouse IgG: RPE
Immunohistochemistry	Recommended Secondary Reagents
	F(ab')2 Rabbit anti-mouse IgG: HRP conjugate
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4j65
Host	Mouse
Source	Chicken
Concentration	1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography from tissue culture supernatant.
Immunogen	Chicken Bursa cells
Specificity	Recognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatability complex (MHC).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.