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You are here:Home » Antibodies » Abs to MHC » Anti -MHC Class II

Anti -MHC Class II

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
During ontogeny the level of B-L expression is reported to increase during the bursal phase of B cell differentiation.
Catalog #M3887-10B1
Fusion PartnersSpleen cells from immunized Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
ApplicationsFlow Cytometry 1:50-1:100
Immunohistochemistry: Frozen  
Immunoprecipitation
Flow Cytometry: Use 10ul of the suggested working dilution to label 10 e6cells in 100ul.
Recommended Secondary ReagentsF(ab')2 Rabbit anti mouse IgG: FITC
F(ab')2 Rabbit anti mouse IgG: RPE
ImmunohistochemistryRecommended Secondary Reagents
F(ab')2 Rabbit anti-mouse IgG: HRP conjugate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4j65
HostMouse
SourceChicken
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography from tissue culture supernatant.
ImmunogenChicken Bursa cells
SpecificityRecognizes a monomorphic determinant on the chicken B-L molecule, the chicken class II major histocompatability complex (MHC).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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