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You are here:Home » Antibodies » Abs to MHC » Anti -MHC II (Histocompatibility-2, MHC, H-2, MHC-II)

Anti -MHC II (Histocompatibility-2, MHC, H-2, MHC-II)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
MHC class II molecules are heterodimers of non-covalently associated alpha (31-34kD) and beta (26-29kD) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II.
Catalog #M3887-10J
The monoclonal antibody is a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells and macrophages) and for studying the development of T helper cells since they stain stromal cells in thymus. The level of antigen detected differs from strain to strain.
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50
Immunohistochemistry (frozen): 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9G231
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenMouse MHC II.
SpecificityRecognizes mouse major histocompatibitity complex class II (MHC class II), molecules that are heterodimers of non-covalently associated alpha (31-34kD) and beta (26-29kD) chains. Species crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesMHC


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