Anti -MHC Class 2 I-a (CD74, DHLAG, Gamma Chain of Class II Antigens, HLA Class II
Histocompatibility Antigen gamma Chain, HLA DR Antigens Associated Invariant Chain, HLA DR
gamma, HLADG, I-a Antigen Associated Invariant Chain, I-a Associated Invariant Chain, I
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of or in association with MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41kD, depending on genetic splicing. | | | Catalog # | M3887-11J | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and purification of Ia antigen from rat spleen. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 1:50-1:100 routinely tested on rat splenocytes | | Immunohistochemistry (Paraffin): 1:50-1:100. Results may be enhanced with heat treatment using 0.01M citrate buffer pH6.0. | | Immunohistochemistry: frozen sections | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8L766 | | Host | Mouse | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Rat thymocyte membrane glycoprotein | | Specificity | Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Species Crossreactivity: certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the rat BDIX strain. Expression is polymorphic in mice | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | CD74 Antigen, CD74 Molecule (Class II Monomorphic) |
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